biotinylated irrelevant antigen Search Results


biotinylated irrelevant antigen  (R&D Systems)
92
R&D Systems biotinylated irrelevant antigen
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Biotinylated Irrelevant Antigen, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/Recombinant+Human+ULBP-2+His-tag+Avi-tag+Protein%2C+CF/pmc11456866-155-39-46
Average 92 stars, based on 1 article reviews
biotinylated irrelevant antigen - by Bioz Stars, 2026-09
92/100 stars
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biotinylated anti-hla-dr  (Immunotec inc)
90
Immunotec inc biotinylated anti-hla-dr
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Biotinylated Anti Hla Dr, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/unconjugated+cd105+antibody/pmc00026365-93-11-13
Average 90 stars, based on 1 article reviews
biotinylated anti-hla-dr - by Bioz Stars, 2026-09
90/100 stars
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antigen  (Sino Biological)
94
Sino Biological antigen
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Antigen, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/Human+CD40+%2F+TNFRSF5+Protein/pmc11456866-130-6-9
Average 94 stars, based on 1 article reviews
antigen - by Bioz Stars, 2026-09
94/100 stars
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biotinylated peptides  (GenScript corporation)
90
GenScript corporation biotinylated peptides
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Biotinylated Peptides, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/biotinylated+peptides/pm37121178-70-3-5
Average 90 stars, based on 1 article reviews
biotinylated peptides - by Bioz Stars, 2026-09
90/100 stars
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magnetic beads invitrogen dynabeads biotin binder no 110 47  (Thermo Fisher)
96
Thermo Fisher magnetic beads invitrogen dynabeads biotin binder no 110 47
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Magnetic Beads Invitrogen Dynabeads Biotin Binder No 110 47, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/Dynabeads+Biotin+Binder/pmc03707688-73-5-7
Average 96 stars, based on 1 article reviews
magnetic beads invitrogen dynabeads biotin binder no 110 47 - by Bioz Stars, 2026-09
96/100 stars
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streptavidin  (Thermo Fisher)
99
Thermo Fisher streptavidin
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/Streptavidin/pmc09542628-441-25-26
Average 99 stars, based on 1 article reviews
streptavidin - by Bioz Stars, 2026-09
99/100 stars
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octet  (Sartorius AG)
99
Sartorius AG octet
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Octet, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/Octet/custom%40octet%4028617578
Average 99 stars, based on 1 article reviews
octet - by Bioz Stars, 2026-09
99/100 stars
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octet bli systems  (Sartorius AG)
99
Sartorius AG octet bli systems
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Octet Bli Systems, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/Octet+BLI+Systems/custom%40octet-bli%4028617578
Average 99 stars, based on 1 article reviews
octet bli systems - by Bioz Stars, 2026-09
99/100 stars
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gr-1-pe  (Becton Dickinson)
90
Becton Dickinson gr-1-pe
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Gr 1 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/ly6g+antibody/pm12884282-123-10-12
Average 90 stars, based on 1 article reviews
gr-1-pe - by Bioz Stars, 2026-09
90/100 stars
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dc-sign-fitc  (Becton Dickinson)
90
Becton Dickinson dc-sign-fitc
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Dc Sign Fitc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/fitc+conjugated+goat+anti+mouse+igg/pmc02562446-32-52-53
Average 90 stars, based on 1 article reviews
dc-sign-fitc - by Bioz Stars, 2026-09
90/100 stars
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multiscreen hts ha elispot plates  (Merck KGaA)
90
Merck KGaA multiscreen hts ha elispot plates
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
Multiscreen Hts Ha Elispot Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/multiscreen+ha+elispot+plates/pmc07580252-109-6-10
Average 90 stars, based on 1 article reviews
multiscreen hts ha elispot plates - by Bioz Stars, 2026-09
90/100 stars
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c antigen  (Miltenyi Biotec)
96
Miltenyi Biotec c antigen
High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to <t>biotinylated</t> SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.
C Antigen, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+irrelevant+antigen/Streptavidin/us11116835-978-51-85
Average 96 stars, based on 1 article reviews
c antigen - by Bioz Stars, 2026-09
96/100 stars
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Image Search Results


High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to biotinylated SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.

Journal: Antibody Therapeutics

Article Title: AI-based antibody discovery platform identifies novel, diverse, and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains

doi: 10.1093/abt/tbae025

Figure Lengend Snippet: High-throughput binding and bioactivity screen allows identification of unique J.HAL® IgG candidates that bind SARS-CoV-2 spike protein and effectively block spike:Human ACE2 receptor interaction. (A and B) Unpurified transfection supernatants from two independent screens were tested by AlphaLISA for binding to biotinylated SARS-CoV-2 spike protein and in parallel to biotinylated irrelevant target protein. Positive control antibody M-3418 (S309) demonstrates binding specificity, whereas isotype control antibody demonstrates lack of binding. Data were graphed using GraphPad prism software. (C and D) Unpurified transfection supernatants from two independent screens were tested for their ability to block binding of SARS-CoV-2 spike protein to human ACE-2 using ELISA. Positive control antibody M-2381 (red symbol) demonstrates effective blockade, whereas isotype control antibody demonstrates lack thereof. Data were graphed as % inhibition +/− SD using GraphPad prism software.

Article Snippet: Briefly, 10 μl of unpurified transfection supernatant diluted 1:200 in 1x Immunoassay buffer (Perkin Elmer cat# AL000F) was preincubated with either 10 μl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549) or with 10 μl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538–050) for 20 min at RT in 96 half-area white microplates (Corning cat# CLS3693).

Techniques: High Throughput Screening Assay, Binding Assay, Blocking Assay, Transfection, Positive Control, Control, Software, Enzyme-linked Immunosorbent Assay, Inhibition

J.HAL® IgG antibodies exhibit dose-dependent binding to SARS-CoV-2 spike protein. Unpurified transfection supernatants were concentration normalized and tested for binding in a 9-point serial titration series to biotinylated SARS-CoV-2 spike protein by AlphaLISA. Representative binding profiles for 30 J.HAL® antibodies are shown in the subplots A-F above, along with the positive control antibody M-3418 (S309). Data were graphed as mean +/− SD using GraphPad prism software.

Journal: Antibody Therapeutics

Article Title: AI-based antibody discovery platform identifies novel, diverse, and pharmacologically active therapeutic antibodies against multiple SARS-CoV-2 strains

doi: 10.1093/abt/tbae025

Figure Lengend Snippet: J.HAL® IgG antibodies exhibit dose-dependent binding to SARS-CoV-2 spike protein. Unpurified transfection supernatants were concentration normalized and tested for binding in a 9-point serial titration series to biotinylated SARS-CoV-2 spike protein by AlphaLISA. Representative binding profiles for 30 J.HAL® antibodies are shown in the subplots A-F above, along with the positive control antibody M-3418 (S309). Data were graphed as mean +/− SD using GraphPad prism software.

Article Snippet: Briefly, 10 μl of unpurified transfection supernatant diluted 1:200 in 1x Immunoassay buffer (Perkin Elmer cat# AL000F) was preincubated with either 10 μl biotinylated SARS-CoV-2 spike protein (3 nM final concentration; R&D Systems cat# BT10549) or with 10 μl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538–050) for 20 min at RT in 96 half-area white microplates (Corning cat# CLS3693).

Techniques: Binding Assay, Transfection, Concentration Assay, Titration, Positive Control, Software